Journal of Applied Electrochemistry 31: 1307-1313, 2001.
2001 Kluwer Academic Publishers. Printed in the Netherlands.
Antioxidant effects of reduced water produced by electrolysis of sodium chloride solutions
K. HANAOKA*
Bio-REDOX Laboratory Inc., 1187-4 Oaza-Ueda, Ueda-shi, Nagano 386-0001, Japan (*fax: +81 268 24 0050)
Received 23 December 2000; accepted in revised form 21 August 2001
= Abstract =
Antioxidant vitamins and enzymes such as superoxide dismutase, catalase and glutathione peroxidase are considered to function as scavengers against reactive oxygen species and to provide protection against reactive oxygen species, including free radicals. Although antioxidants such as L-ascorbic acid, d-catechin and quercetin dehydrate show superoxide dismutation activity, using reduced water produced in the cathode side by electrolysis as a solvent instead of 2 mM NaC1 solution of the same pH level as the reduced water increased the superoxide dismutation activity of these antioxidants. Moreover, neither the reduced water nor its electrolyte solution showed any superoxide dismutation activity by itself. On the other hand, the reduced water was able to decrease hydrogen peroxide levels. It has been found that the behaviour of H2 in reduced water, which was activated by a platinum
electrode, differed from that of H2 introduced by bubbling of hydrogen gas. The former decreased H2O2, whereas the latter did not. These results suggest strongly that the increase in superoxide dismutation activity, with a proton donor such as L-ascorbic acid, is due to an increase in the dissociation activity of water while the scavenging activity for H2O2 is due to activated dissolved H2 in the reduced water.
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